The status of Acanthochitona pilosa Schmidt-Petersen, Schwabe & Haszprunar, 2015 as a distinct species is firmly confirmed by additional discriminating morphological characters and genetic analysis. Furthermore, new information has become available on its habitat, biology, and distribution range. The genetic analysis also shows the urgent need for a more comprehensive, large scale molecular phylogeny and species delimitation of European / North African Acanthochitonidae.